Nucleus Segmenter

Identify nuclei in a microscopy image and assign each pixel of the image to a particular nucleus




This model detects nuclei in a microscopy image and specifies the pixels in the image that are assigned to each nucleus. The model is developed based on the architecture of Mask R-CNN using Feature Pyramid network (FPN) and a ResNet50 backbone. Given an image (of size 64 x 64, 128 x 128 or 256 x 256), this model outputs the segmentation masks and probabilities for each detected nucleus. The mask is compressed using Run-length encoding (RLE).

The model is based on the TF implementation of Mask R-CNN. The model is trained on the Broad Bioimage Benchmark Collection (Accession number BBBC038, Version 1) dataset of annotated biological images.


He, K., Gkioxari, G., Dollár, P. and Girshick, R., 2017, October. Mask R-CNN. In Computer Vision (ICCV), 2017 IEEE International Conference on (pp. 2980-2988). IEEE.Ljosa, V., Sokolnicki, K.L. and Carpenter, A.E., 2012. Annotated high-throughput microscopy image sets for validation. Nature methods, 9(7), pp.637-637.Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012].Mask R-CNN Github Repository

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